Reading this material and answering the questions at the end of each. Schedule time on a cytometer facs or calibur by filling in the calendar at the flow lab. Other applications flow cytometry is indicated in the evaluation of serous effusions and csf, including aqueous or vitreous humor of patients with a history of hematolymphoid neoplasia. The bd facscanto is often hailed as the new generation of flow cytometry. This lets you run the applications you want to run, while enjoying the automation benefits that aquios can provide. Bd facscalibur flow cytometer from bd biosciences selectscience. Not for use in diagnostic or therapeutic procedures. Bd facscalibur instructions for use manual pdf download. The bd facscanto flow cytometer reference manual assumes you have a. Designed specifically to support a wide range of applications, the. It designed for applications that range from routine clinical to advanced research, analyzing single cells as they pass through a focused laser beam. Fill the sheath fluid reservoir of the cytometer with pbs before getting started.
Bd facscan anaylser at flow cytometry core facility. Bd facsdiva software features specific to the bd facscanto cytometer within this manual. One striking difference between the facscanto and older generation machines such as the facscalibur is that this system operates on a pcbased windows xp platform using bd facsdiva software. Bd facscalibur, bd facscan, bd facsort, or bd lsr flow cytometer. It analyzes cells as they pass through a focused laser beam one at a time in a moving. Becton dickinson facscalibur flow cytometry system youtube.
I will look at difference on a celltocell level and thus use a flow cytometer bd accuri c6. It is a tricolor, automated system that is capable of analyzing a wide variety of. Combined with your choice of sample loader, a wide variety of reagents, and powerful software, the bd facscalibur. An optional, auto mated sample loader is also available. Flow cytometry is a rapid and useful method of obtaining a diagnosis. Becton dickinson bd facscalibur flow cytometer tags. Flow cytometry analysis using the becton dickinson facs calibur. Biomedical and biological sciences 44,549 views 17. We have 1 bd facscalibur manual available for free pdf download.
Figure 11 bd facscalibur system with bd facs loader option. Bd facscanto ii instructions for use manual pdf download. Flow cytometry analysis using the becton dickinson facs. The bd facscalibur platform allows users to perform both cell analysis and cell sorting in a single benchtop system. Bd biosciences accuri c6 personal flow cytometer features. If communication problems exist between the loader and cytometer, check cable connections and ensure the correct port is selected in loader manager preferences. The hypothesis is that at lower growth rate, the 6. The gold standard that continues to evolve the bd facscalibur flow cytometer is another bd biosciences first. A bd facscan system the facscan system is an automated. Bd facscalibur startup and cleanup procedures video at. Ask bd email updates contact info website feedback. Bd facscalibur, and bd lsr cytometers and streaminair cytometers.
The compact footprint, portable weight, and rugged design of the bd accuri c6 plus also make it a valuable personal use tool for experienced researchers who want a cytometer to be easily. Guide to using bd cba kits with the bd facscalibur system. In contrast, a cytometer can measure 20 30 k cellssec. View and download bd facscalibur instructions for use manual online.
The bd facscanto ii instructions for use assumes you have a working knowledge of basic microsoft windows operation. The system supports a wide variety of research and clinical applications and is complemented by a broad suite of intuitive software solutions to streamline analysis for a wide range of applications including enumeration of. Bd biosciences recommends that firsttime users of this instrument take advantage of operator training offered with the sale of every new instrument. Autoflorescence background compensation setup application of fcm basic principle of flow cytometry bd facscalibur flow cytometry analyzer. Convenient qc updates compensation values and ensures the flow cytometer is performing within specifications, without the need for manual adjustments. Dec 03, 2014 flow cytometry is a rapid and useful method of obtaining a diagnosis. There is also a second power toggle switch hidden on the back of the machine. Aug 10, 2017 the principle of flow cytometry and facs 2 facs. Discover clinical simplicity with the bd facsvia flow. This guide should help you operating the flow cytometer and leaving it such way that another colleague can use it another day. The aquios designer software allows customers to create user defined protocols, compensation matrix, sample preparation steps, and qc features on the aquios cl flow cytometer.
Based on keywords that are entered prior to acquisition and read by fcap array software, analysis begins as soon as the fcs data files are identified. Intuitive software for accurate and fast results simple to install, learn and use, the softwares preconfigured assay templates minimize training time and expense. Service for bd accuri c6 systems and their related options will continue until december 31 st, 2021. The beckman calibur cytometers have a green power button on the right side. The accuri c6 flow cytometer a small revolution request pdf. As a modular option for new or existing bd facscalibur flow cytometers, the hts option is designed to speed through a variety of microtiter plates in less than 15 minutes and support a wide range of applications including the bd cytometric bead array assay.
It is a tricolor, automated system that is capable of. The hypothesis is that at lower growth rate, the 6 below stated traits should vary more. Users of the bd facsverse flow cytometer equipped with bd facsuite software will enjoy many automated features for even faster results. Bd accuri c6 system 32bit software is not forward compatible with bd accuri c6 plus 64bit software and the fcs 3. Request a quote for a bd facscelesta flow cytometer. Bd biosciences provides flow cytometers, reagents, tools, and a wide range of services to support the work of researchers and clinicians who understand disease and improve care. Fitc and alexa fluor 488 fl1 channelpe fl2percp, pecy5, percpcy5.
Facs flow cytometry is a technique used to measure the physical and chemical properties of cells orthe physical and chemical properties of cells or. Facscanto flow cytometer from bd biocompare product. Bd facscalibur instructions for use bd biosciencesus. Documents document view page bd facscalibur cytometer. Unless otherwise noted, bd, the bd logo and all other trademarks are property of becton, dickinson and company.
Current extended warranties will not be affected and will be supported until they expire. Instruments available for use at the flow cytometry core facility, institute of cell and molecular science, barts and the london, queen mary, university of london. The facscalibur system is a modular, benchtop flow cytometer from becton dickinson immunocytometry system bdis that is easily modified based on research needs. Instructions for use manual bd facscalibur instructions for use manual 238 pages. Facscalibur measuring instruments pdf manual download. Request pdf on may 31, 2010, collin rich and others published the accuri c6 flow cytometer a small revolution find, read and cite all the research you need on researchgate. The bd facscalibur system is an automated benchtop flow cytometry system that offers three or fourcolor capability and can perform both cellular analysis and optional cell sorting.
Benchtop flow cytometers bd facscaliburbd facscalibur. From the drop down menu under cytometer, select the fluidics shutdown option. Bd facs calibur microscopy and microanalysis facility. The facs calibur replaces both the facscan and facsort. Similarly, comparison of stemkitversus sce kit on the bd facscalibur generated an r 2 value of 0. Flow cytometry is a technology that simultaneously measures and then analyzes multiple physical characteristics of single particles, usually cells, as they. Purpose of this guide who should read this guide this guide provides setup and acquisition instructions for using a bd facscalibur flow cytometer single or dual laser to acquire data obtained with a. The becton dickinson facscalibur flow cytometer is an innovative single benchtop system, combining cell analysis and employing a customizable approach to flow cytometry analysis through modular components. The intuitive bd facscalibur flow cytometer instrument can be upgraded to keep up with the laboratorys sorting and automation needs. Bd facscalibur instructions for use verifying connection ports the loader electronics module connects to the computer via a keyspan usbtoserial adapter. Finally, comparison of the stemkit on the bd facscalibur versus sce kit on the bd facscanto generated an r 2 value of 0. May, 2016 becton dickinson facscalibur flow cytometry system with hts autosampler available at the lab world group.
A cellquest users guide pdf is on the computer desktop feel free to copy it onto a usb drive if you want to browse through it at your leisure and contains much more information on the software than. Reading this material and answering the questions at the. The cell or particle scatters the light into forward and side scatter signals indicators of the particles size, shape and structure. The operation of the becton dickinson facs calibur and cellquest software version 3.
Bd biosciences provides flow cytometers, reagents, tools and a wide range of services to support the work of researchers and clinicians who understand disease and improve care. Bectondickinson facscalibur flow cytometer instructions. Bd biosciences recommends that firsttime users of this cytometer take advantage of operator training offered with the sale of every new cytometer. Boot the computer after the cytometer is turned on otherwise the bd startup init wont find the cytometer. Video called bd facscalibur startup and cleanup procedures, tagged as cytometer cleaning procedure, cytometer start up, cytometer shut down. All reagents and kits are compatible with both bd accuri c6 plus and accuri c6 flow cytometer systems.
100 56 931 342 711 1470 883 471 75 1051 903 101 1014 929 1053 1383 1464 840 527 1090 109 1426 915 1117 660 1550 1052 497 156 217 112 1469 1539 154 1562 754 1308 1148 1434 928 1124 308 525